Mouse T Cell Enrichment Kit

A single cell suspension from pooled c57bl 6 mouse peripheral lymphoid tissues was prepared to isolate cd8 t cells using the mojosort mouse cd8 t cell isolation kit.

Mouse t cell enrichment kit.

Easysep mouse cd4 t cell isolation kit catalog 19852. Cells were stained with cd8 clone 53 6 7 pe and cd3ε clone 145 2c11 apc. T cell enrichment column kits are also available to isolate specific t cell subpopulations of interest. Easysep mouse t cell isolation kit catalog 19851 easysep mouse t cell isolation cocktail 0 5 ml.

Before loading onto t cell subset columns leukocyte suspensions are incubated with a mixture of monoclonal antibodies. Unwanted cells are targeted for removal with tetrameric antibody complexes recognizing non t cells and dextran coated magnetic particles. Fast and easy to use up to 99 purity no columns required untouched viable cells. Designed to prepare purified murine t cell populations via high affinity negative selection.

The magnisort mouse t cell enrichment kit is designed for the magnetic separation of mouse t cells by negative selection. Monoclonal antibody cocktails are designed to recognize undesired t cells and other undesired cell populations. This product replaces the easysep mouse cd4 t cell enrichment kit catalog 19752 for even faster cell isolations. Unwanted cells are targeted for removal with tetrameric antibody complexes recognizing non cd8 t cells and glycophorin a.

Fast and easy to use up to 96 purity no columns required untouched viable cells. The cd4 t cell isolation kit mouse was developed for the untouched isolation of cd4 t cells from single cell suspensions of mouse spleen and or lymph nodes. Add the bioinylated mouse cd8 t lymphocyte enrichment cocktail at 5 µl per 1 x 10 6 cells and incubate on ice for 15 minutes 5. The easysep human t cell enrichment kit is designed to isolate t cells from fresh or previously frozen peripheral blood mononuclear cells by negative selection.

This kit uses the new fast mouse protocol and replaces the cd4 t cell isolation kit ii 130 095 248 allowing isolation of cd4 t cells in just 18 minutes. This product replaces the easysep mouse t cell enrichment kit catalog 19751 for even faster cell isolations. Available for human mouse and rat cells purifies cd3 t cells to 81 88 based upon gravitational flow simple procedure. If cells are more dilute than 10 x 10 6 cells ml then spin down the cells and resuspend them in tissue culture medium or 1x bd imag buffer at a concentration of 20 x 10 6 cells ml.